Abstract
Sulfurospirillum multivorans is a free-living, physiologically versatile Epsilonproteobacterium able to couple the reductive dehalogenation of chlorinated and brominated ethenes to growth (organohalide respiration). We present proteomic data of S. multivorans grown with different electron donors (formate or pyruvate) and electron acceptors (fumarate, nitrate, or tetrachloroethene [PCE]). To obtain information on the cellular localization of proteins, membrane extracts and soluble fractions were separated before data collection from both fractions. The proteome analysis of S. multivorans was performed by mass spectrometry (nanoLC-MS/MS). Raw data have been deposited at ProteomeXchange, “ProteomeXchange provides globally coordinated proteomics data submission and dissemination” [1], via the PRIDE partner repository with the dataset identifier PRIDE: PXD004011. The data might support further research in organohalide respiration and in the general metabolism of free-living Epsilonproteobacteria. The dataset is associated with a previously published study “Proteomics of the organohalide-respiring Epsilonproteobacterium S. multivorans adapted to tetrachloroethene and other energy substrates” [2].
Highlights
Proteomic data set of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates
We present proteomic data of S. multivorans grown with different electron donors and electron acceptors
We present the proteome of the organohalide-respiring, free-living Epsilonproteobacterium Sulfurospirillum multivorans grown with different electron donors and electron acceptors
Summary
The defined mineral medium used for growth of S. multivorans (German Collection of Microorganisms [DSMZ] number 12446) was prepared as previously described [4]. Precultures for inoculation of the main cultures were grown in rubber stoppered 200 mL serum bottles; transfers were always performed with 10% culture volume. All precultures were inoculated with cultures grown with pyruvate and fumarate for 60 transfer steps to generate cells lacking proteins involved in organohalide respiration [3]. Before inoculation of the main culture, the precultures were transferred three consecutive times into the desired medium with a 10% inoculum in order to avoid transfer of pyruvate or fumarate from the initial preculture. The ratio between medium and gas phase (v/v) during all growth experiments was 1 to 1. Six electron donor/acceptor conditions were used: the standard condition pyruvate/fumarate (Py/Fu) and five conditions for comparison: pyruvate/nitrate (Py/Ni), pyruvate/PCE (Py/PCE), formate/fumarate (Fo/ Fu), formate/nitrate (Fo/Ni) and formate/PCE (Fo/PCE)
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