Abstract
Yiqi Fumai lyophilized injection (YQFM) is the recombination of Sheng mai san (SMS).YQFM has been applied clinically to efficaciously and safely treat chronic heart failure (CHF). However, the mechanism of YQFM is still not fully elucidated. The purpose of our study was to investigate the protective mechanism of YQFM against abdominal aortic coarctation (AAC) in rats by proteomic methods. After YQFM treatment, the cardiac function were obviously meliorated. One hundred and fifty-seven important differentially expressed proteins (DEPs) were identified, including 109 in model rat compared with that in control rat (M:C) and 48 in YQFM-treated rat compared with that in model rat (T:M) by iTRAQ technology to analyze the proteomic characteristics of heart tissue. Bioinformatics analysis showed that DEPs was mainly involved in the body’s energy metabolism and was closely related to oxidative phosphorylation. YQFM had also displayed efficient mitochondrial dysfunction alleviation properties in hydrogen peroxide (H2O2)-induced cardiomyocyte damage by Transmission Electron Microscope (TEM), Metabolic assay, and Mitotracker staining. What’s more, the levels of total cardiomyocyte apoptosis were markedly reduced following YQFM treatment. Furthermore, Western blot analysis showed that the expressions of peroxisome proliferator activated receptor co-activator-1α(PGC-1α) (p < 0.01 or p < 0.001), perixisome proliferation-activated receptor alpha (PPAR-α) (p < 0.001)and retinoid X receptor alpha (RXR-α) were upregulated (p < 0.001), PGC-1α as well as its downstream effectors were also found to be upregulated in cardiomyocytes after YQFM treatment(p < 0.001).These results provided evidence that YQFM could enhance mitochondrial function of cardiomyocytes to play a role in the treatment of CHF by regulating mitochondrial biogenesis-related proteins.
Highlights
Chronic heart failure is myocardial damage caused by various reasons, resulting in injures the function and structure of the myocardium, and resulting in low ventricular pumping or filling function (Mosterd and Hoes, 2007)
In order to detect the chemical constituents of Yiqi Fumai lyophilized injection (YQFM), an high performance liquid chromatography (HPLC) method was established
In Chronic heart failure (CHF) rat administered YQFM left ventricular rejection fraction (LVEF)% and left ventricular fractional Shortening (LVFS)% were effectively restored to normal levels, respectively (Figures 2B,C)
Summary
Chronic heart failure is myocardial damage caused by various reasons, resulting in injures the function and structure of the myocardium, and resulting in low ventricular pumping or filling function (Mosterd and Hoes, 2007). The mortality rate of patients with CHF(4-year) is up to 50%, and the mortality rate of patients (1-year) with severe heart failure is as high as 50% (Heo et al, 2008; Yancy et al, 2013; Wang et al, 2017). Targeting the synchrony of ventricular function, these drugs can alleviate patient mortality and relieve symptoms with advantage. There are some adverse events that may limit the use of TCM (Fu et al, 2010; Faris et al, 2012; Wang et al, 2015a), so conventional treatment of western medicine is often combined with TCM syndrome differentiation and treatment, and the advantages of Chinese and Western medicine are complemented
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