Proteomic Analysis Reveals Growth Inhibition of Coriolus versicolor by Methanol Extracts of Cinnamomum camphora Xylem

Quan Li,Hui Lin,Xiangyang Li,Zhe Wang

doi:10.1155/2021/6337906

Quan Li, Hui Lin + Show 2 more

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https://doi.org/10.1155/2021/6337906

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Abstract

The extracts of decay-resistant tree species are important research objects for the future development of wood preservatives. To understand the antifungal mechanisms of Coriolus versicolor inhibition with methanol extracts of C. camphora xylem, the protein profiles of C. versicolor were analyzed using 2-DE followed by MALDI-TOF/MS and bioinformatic analyses. The results showed that 41 protein spots were obviously changed among the 366-385 protein spots of C. versicolor treated with methanol extracts of C. camphora xylem. Twenty-one protein spots were upregulated, and 20 protein spots were downregulated. Cellular localization was performed to identify these differential proteins, and biological process and functional analysis found that 9 of these proteins were in the cytoplasm, 6 were intracellular, and 5 were in the mitochondrion. A total of 18.8% were mapped to small-molecule metabolic processes, 12.5% to cellular amino acid metabolic processes, and 10.9% to cellular nitrogen compound metabolic processes. Twenty-five percent of the differential proteins were associated with ion bonding, 15% with oxidoreductase activity, and 15% with ATPase activity and transmembrane transport activity. Downregulated expression of aspartate aminotransferase, ATP synthase alpha chain, DEAD/DEAH-box helicase, and phosphoglycerate kinase showed that the methanol extracts of C. camphora xylem disrupted functional aspects such as nitrogen and carbon metabolism, energy metabolism, hormone signal response, and glucose metabolism, eventually leading to C. versicolor inhibition.

Highlights

Wood is an economical and environmentally friendly material and which is used for many end uses, such as furniture, wood-based panels, and wooden structures
The results which the 2D-GE diagram of C. versicolor treated with methanol extracts of C. camphora xylem can be seen in Figure 1, which shows that the two-dimensional electrophoretogram spectral patterns of the two proteins are basically the same, and the protein spots are mainly concentrated in the pH range of 4–7
The research reveals the antifungal mechanism of the secondary metabolites of C. camphora xylem in methanolic extracts through 26]. Twodimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF-MS

Summary

Introduction

Wood is an economical and environmentally friendly material and which is used for many end uses, such as furniture, wood-based panels, and wooden structures. Many types of wood have natural decay resistance properties. In addition to the different biological structures of wood, the academic community has agreed that there are differences in the chemical composition and contents of wood. These differences in chemical compositions are one of the important factors leading to variation in natural decay resistance performance [5]. These studies usually focused on antibacterial, decay resistance, and antioxidant compounds and their application [6, 7]. The application of botanical extracts in wood preservative impregnation is one new technique in the wood science field

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