Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t

Dagang Tian,Songbiao Chen,Ziqiang Chen,Feng Wang,Zaijie Chen,Liming Yang,Yuanchang Zhou,Liu Yang,Yuming Luo

doi:10.1186/s12284-018-0240-3

Abstract

BackgroundRice blast (caused by Magnaporthe oryzae) is one of the most destructive diseases of rice. While many blast resistance (R) genes have been identified and deployed in rice cultivars, little is known about the R gene-mediated defense mechanism. We used a rice transgenic line harboring the resistance gene Piz-t to investigate the R gene-mediated resistance response to infection.ResultsWe conducted comparative proteome profiling of the Piz-t transgenic Nipponbare line (NPB-Piz-t) and wild-type Nipponbare (NPB) inoculated with M. oryzae at 24, 48, 72 h post-inoculation (hpi) using isobaric tags for relative and absolute quantification (iTRAQ) analysis. Comparative analysis of the response of NPB-Piz-t to the avirulent isolate KJ201 and the virulent isolate RB22 identified 114 differentially expressed proteins (DEPs) between KJ201-inoculated NPB-Piz-t (KJ201-Piz-t) and mock-treated NPB-Piz-t (Mock-Piz-t), and 118 DEPs between RB22-inoculated NPB-Piz-t (RB22-Piz-t) and Mock-Piz-t. Among the DEPs, 56 occurred commonly in comparisons KJ201-Piz-t/Mock-Piz-t and RB22-Piz-t/Mock-Piz-t. In a comparison of the responses of NPB and NPB-Piz-t to isolate KJ201, 93 DEPs between KJ201-Piz-t and KJ201-NPB were identified. DEPs in comparisons KJ201-Piz-t/Mock-Piz-t, RB22-Piz-t/Mock-Piz-t and KJ201-Piz-t/KJ201-NPB contained a number of proteins that may be involved in rice response to pathogens, including pathogenesis-related (PR) proteins, hormonal regulation-related proteins, defense and stress response-related proteins, receptor-like kinase, and cytochrome P450. Comparative analysis further identified 7 common DEPs between the comparisons KJ201-Piz-t/KJ201-NPB and KJ201-Piz-t/RB22-Piz-t, including alcohol dehydrogenase I, receptor-like protein kinase, endochitinase, similar to rubisco large subunit, NADP-dependent malic enzyme, and two hypothetical proteins.ConclusionsOur results provide a valuable resource for discovery of complex protein networks involved in the resistance response of rice to blast fungus.

Highlights

Rice blast is one of the most destructive diseases of rice
Disease assessment and isobaric tags for relative and absolute quantification (iTRAQ) analysis To compare the responses of wild-type Nipponbare (NPB) and Nipponbare harboring the Piz-t gene (NPB-Piz-t) to infection with avirulent and virulent M. oryzae isolates, we inoculated NPB and NPB-Piz-t with isolates KJ201 and RB22, respectively
Proteins extracted from leaves of NPB and NPB-Piz-t inoculated with M. oryzae isolates were subjected to iTRAQ analysis

Summary

Introduction

Rice blast (caused by Magnaporthe oryzae) is one of the most destructive diseases of rice. High-throughput gene and protein profiling techniques have enabled investigation of the molecular processes involved in the defense responses of rice to M. oryzae infection (Li et al 2006; Vergne et al 2007, 2010; Bagnaresi et al 2012; Gupta et al 2012; Kim et al 2013; Li et al 2014, 2015; Zhang et al 2016; Jain et al 2017). These large-scale transcriptomic and proteomic analyses have identified rice genes and/or proteins that were differentially expressed upon infection by M. oryzae and provided overviews of biological processes characterizing rice-M. oryzae interactions

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