Abstract
BackgroundExosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes. The aim of this study was to characterize the proteomic content of lymphoma cell-derived exosomes (LCEXs).MethodsIn this study, exosomes derived from Raji cells (EXORaji) were purified and proteins of EXORaji were separated by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein bands were identified by mass spectrometry. The protein components of EXORaji were analyzed using shotgun technology, and the function proteins of EXORaji were defined and described using the Gene Ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.ResultsA total of 197 proteins were identified in EXORaji; 139 proteins were also identified in Raji cells, showing an overlap of 70.56% of the total proteins in EXORaji. Interestingly, the remaining 58 proteins were unique to EXORaji. The GO database and KEGG were used to define and describe the function of proteins. The data showed that some important proteins involved in antigen procession and presentation as well as cell migration and adhesion were also identified in EXORaji, such as MHC-I and II, HSC70, HSP90, and ICMA-1.ConclusionsLCEXs express a discrete set of proteins involved in antigen presentation and cell migration and adhesion, suggesting that LCEXs play an important role in the regulation of immunity and interaction between lymphoma cells and their microenvironment. LCEXs harbor most of the proteins of lymphoma cells and could be one of the sources of lymphoma-associated antigens for immunotherapeutic purposes.
Highlights
Exosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes
Molecules involved in antigen presentation such as major histocompatibility complex (MHC)-I and MHC-II have been detectable in dendritic cells (DCs)-derived exosomes (DEXs) [6,7]
ER-residing molecules such as ER-residing protein Grp94 in exosomes derived from Raji cells were examined by Western blot
Summary
Exosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes. The aim of this study was to characterize the proteomic content of lymphoma cell-derived exosomes (LCEXs). Exosomes are a type of vesicle secreted by late endosomes in eukaryotic cells and loaded with cell membrane molecules, microRNAs, and proteins [1,2,3,4]. Studies on the protein composition of exosomes of dendritic cells (DCs) showed that loaded proteins are mainly involved in regulation of cell physiological activities [5,6,7]. Molecules involved in antigen presentation such as major histocompatibility complex (MHC)-I and MHC-II have been detectable in DC-derived exosomes (DEXs) [6,7]. DEXs can induce antigen-specific antitumor immunity, the biological characteristics of tumor cellderived exosomes (TEXs), especially those from lymphoma. BL tumor cells were chosen as a model system to study the protein components of exosomes derived from human lymphoma cells because few data are available on the protein components of exosomes derived from this tumor type
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