Protein Tyrosine Nitration during Development and Abiotic Stress Response in Plants.

Capilla Mata-Pérez,Mounira Chaki,María N Padilla,Juan C Begara-Morales,Raquel Valderrama,Francisco J Corpas,Beatriz Sánchez-Calvo,Juan B Barroso

doi:10.3389/fpls.2016.01699

Abstract

In recent years, the study of nitric oxide (NO) in plant systems has attracted the attention of many researchers. A growing number of investigations have shown the significance of NO as a signal molecule or as a molecule involved in the response against (a)biotic processes. NO can be responsible of the post-translational modifications (NO-PTM) of target proteins by mechanisms such as the nitration of tyrosine residues. The study of protein tyrosine nitration during development and under biotic and adverse environmental conditions has increased in the last decade; nevertheless, there is also an endogenous nitration which seems to have regulatory functions. Moreover, the advance in proteome techniques has enabled the identification of new nitrated proteins, showing the high variability among plant organs, development stage and species. Finally, it may be important to discern between a widespread protein nitration because of greater RNS content, and the specific nitration of key targets which could affect cell-signaling processes. In view of the above point, we present a mini-review that offers an update about the endogenous protein tyrosine nitration, during plant development and under several abiotic stress conditions.

Highlights

Nitric oxide (NO) is a short-lived gaseous free-radical molecule with high chemical reactivity and diffusion capacity that can mediate most biological actions in which nitric oxide (NO) is involved
We present a mini-review that offers an update about the endogenous protein tyrosine nitration, during plant development and under several abiotic stress conditions
These findings suggest that salt stress promotes a NO release from peroxisomes to the cytosol for the generation of ONOO−, which is involved in protein tyrosine nitration and provokes nitrosative stress

Summary

Introduction

Nitric oxide (NO) is a short-lived gaseous free-radical molecule with high chemical reactivity and diffusion capacity that can mediate most biological actions in which NO is involved. This PTM seems to be mediated by ONOO−, and this indicates that a boost in the number of proteins or an intensification of specific proteins resulting from tyrosine nitration could be considered an indicator of nitrosative stress in plants (Corpas et al, 2007; Corpas et al, 2009a), as has been demonstrated in animal cells. Most investigations on protein tyrosine nitration in plants have focused on the analysis of this process under abiotic stress situations.

Results

Conclusion