Abstract
Complete cerebral ischemia for 30 min was produced in normothermic cats by clamping the innominate and the left subclavian arteries and simultaneous lowering of the systemic blood pressure. Recovery of neuronal function after ischemia was ascertained by recording the EEG and the pyramidal response to electrical stimulation of the sensorimotor cortex. The incorporation of [ 14Clabeled amino acids into brain proteins was determined at different time intervals after ischemia. Specific radioactivity of brain proteins approached control values within 4 h. At the same time, however, the specific radioactivity of total amino acids in the free pool of the brain was considerably increased. Sucrose density gradient analysis and electron microscopic studies revealed a distribution of polysomes and monosomes during ischemia similar to that in control animals, but a marked disaggregation of polysomes was observed in the postischemic recovery phase. The data are discussed with regard to the possible mechanism of inhibition of cerebral protein synthesis during and after ischemia.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call