Abstract
Nuclei from nerve, astrocytic and other glial cells were purified in hypertonic sucrose and their protein synthesis investigated under carefully optimized conditions of incubation. The incorporation of labelled amino acid into the proteins of brain nuclei and of liver nuclei treated in the same way, proceeded linearly with time for nearly 2 h, the former being about 3 times more active than the latter. After fractionation of the brain nuclei into the different morphological types, linearity ceased after 30–45 min. The incorporation into neuronal nuclei was 1.8 times lower than in astrocytic nuclei, and 1.6 times higher than in nuclei from oligodendro- and microglial cells. Triton X-100 inhibited completely the nuclear protein synthesis. The possible interference of cytoplasmic contaminants in this activity is discussed and dismissed.
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