Protein, sulphydryl, and disulphide concentrations in plasma and in liver and kidney supernatants from rats on low-and high-selenium diets

Abstract

Abstract Liver supernatants from rats on diets deficient in selenium contained significantly more protein than did supernatants from rats on diets supplemented with selenium. Molecules of low molecular weight separated by gel filtration from liver supernatants of high-selenium animals contained significantly more free sulphydryl groups than did samples from low-selenium animals. The in vitro uptake of 75Se-sodium selenite into liver supernatant does not require the presence of small molecules. Peaks of 75Se activity in proteins from liver supernatants separated on Sephadex G-200 did not correlate with peaks of sulphydryl or disulphide activity.