Abstract
Abstract Rats were injected with a mixture of 75Se-selenomethionine and 35S-methionine. The distribution of 75Se and 35S in tissues and proteins of plasma, liver supernatants, and kidney supernatants was studied after 1 day and 3 days. Measurement of the 75Se/35S ratios for a number of tissues showed that blood and kidneys at 1 day and blood, kidneys, and pancreas at 3 days contained more 75Se than 35S (75Se/35S > 1.7). In all other tissues this ratio varied between 0.6 and 1.1. Gel filtration of liver and kidney supernatants showed that the small molecules contained more 35S than 75Se; moreover the distribution of 35S and 75Se within the proteins themselves was quite different, particularly in proteins of intermediate molecular weight. Whereas most of the plasma proteins separated by gel filtration or cellulose acetate electrophoresis contained some 35S activity, only one main peak of 75Se was present and this migrated with the α-globulins on cellulose acetate and before the albumin on Sephadex G-200. That 75Se and 35S were not present in proteins in exactly the same form was confirmed by dialysing proteins against a pH 11.4 buffer, when more 75Se than 35S was released from all samples.
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