Abstract
Force curve measurement by atomic force microscopy has begun to establish its usefulness as a new tool for the investigation of the physical properties of biological materials and processes. Since directly observable quantity in such experiments is the magnitude of the deflection of the cantilever in response to the force exerted on it, it is often difficult to distinguish specific sample responses that are to be measured from superfluous ones due to unwanted interactions between the atomic force microscope (AFM) tip and the sample, or the substrate. In this study, we analyzed force curves obtained in our laboratory over protein samples and tried to extract vital information on the mechanical strength of proteins under different conditions.
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