Protein Staining with Calconcarboxylic Acid in Polyacrylamide Gels

Abstract

AbstractSodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has become a highly reliable separation technique for protein characterization. Broad application of electrophoresis techniques has required the development of detection methods that can be used to visualize the proteins separated on polyacrylamide gels. A few of these methods are Coomassie blue (CB) staining (1), silver staining (2), fluorescent staining (3–5), specific enzyme visualization (6), and radioactive detection (7).CB staining is the most commonly used method owing to its proven reliability, simpliCity>, and economy (8, 9), but it lacks sensitivity compared with the silver-staining method. In addition, the staining/destaining process is time consuming (10). Silver staining is the most sensitive nonradioactive protein detection method currently available, and can detect as little as 10 fg of protein (11, 12). However, it has several drawbacks, such as high-background staining, multiple steps, high cost of reagent, and toxicity of formaldehyde (10).KeywordsSilver StainingCoomassie BlueCoomassie Blue StainingMethanol ContentRadioactive DetectionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.