Abstract
In this study an enhancement methodology, which utilizes time dependent growth of immobilized gold nanoparticles (GNPs) for LSPR-based biosensor platform was developed. The chrono-growth methodology was used for protein analysis and quantification. The method consisted GNP immobilization onto well-plates, GNP chrono-growth, and antibody functionalization. Success of each step was verified by UV–Vis spectrum measurement. Afterwards, the biosensor platform was tested to determine its characteristics. Bovine Serum Albumin (BSA) was chosen to be used as a model protein and an LoD value of 0.344 µM and a dynamic detection range of 1 to 1000 µM was calculated. The results were acquired within 30 min. Developed platform provides simple and rapid detection of the protein.
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