Abstract
Human semen from 79 males was examined morphologically and divided in 5 groups according to the quality. The groups were compared using the following parameters: total protein, total lactate dehydrogenase activity, disc electrophoresis, and quantitation of the proteins and LDH isoenzymes present in the seminal plasma. Concerning total protein, total LDH activity and fractionation of the proteins no information was given which could support the attempts to evaluate fertility. The LDH isoenzymes showed, in addition to the five isoenzymes known from human serum, an extra fraction (LDH x) situated between LDH III and LDH IV. This isoenzyme was only demonstrated in connection with the presence of sperms and was consistently absent in aspermia. The LDH x ratio of total LDH decreased from the group with normal fertility to the group with very low fertility. In the group with sperm survival of less than 48 h, we found that the ratio of LDH x to total LDH was highest, compared with the other groups.
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