Protein Electrophoretogram in Serum of Acute Ischemic Stroke Patients & Its Correlation with S-100 ββ and Neuron Specific Enolase Level: A Pilot Study

Abstract

Attempts are being made to find out the role of biochemical markers in the serum of acute ischemic stroke (AIS) patients, which could be of help in monitoring disease. In the present study we performed electrophoretogram (nondenaturing Polyacrylamide gel electrophoresis) of serum samples obtained from AIS patients at 0, 24, 48, 72 and 144 hours after onset of symptoms and compared with S-100 ββ protein and neuron specific enolase (NSE). We have identified one particular protein band with high molecular weight of >97KD in serum of healthy subjects which was initially absent in the AIS patients and reappeared with the treatment. This HMWP correlated well with the S-100 ββ and NSE protein findings in the same serum samples. S-100 ββ was detected in none of the matched control subjects, NSE level in the normal individuals was found to be 12ng/ml. High expression of these proteins were observed in 24–48hrs sample of the AIS patients and returned to the normal level with the treatment. There was significant correlation observed between the protein band and S-100 ββ and NSE expression. Detection of marker protein in serum samples could be useful for the diagnosis and prognosis of AIS. doi: 10.5214/ans.0972.7531.2006.130203