Abstract
Protein aggregation plays a crucial role in amyloid lesions but it is a huge challenge to monitor the slight changing process dynamically. Here, we propose an optofluidic surface-enhanced Raman scattering (SERS) sensing chip. It consists of several microdisks deposited with gold nanoparticles (Au NPs), where the microdisk acts as a whispering gallery microcavity (WGM) for light confinement and the Au NPs generate the localized surface plasma for electromagnetic enhancement. The effects of different stimulus factors on the secondary structure of lysozyme protein are revealed by observing the change of Raman spectroscopy of amide band with the enhancement of light-matter interaction. The optofluidic SERS design illuminates the potential for dynamic sensing of biological fluid and cellular microenvironment on chip.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Colloids and Surfaces A: Physicochemical and Engineering Aspects
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
