Abstract
Background and Aims: Methotrexate (MTX), a folic acid antagonist, is widely used as a treatment for malignancies as well as in the treatment of various inflammatory and autoimmune disorders. Nephrotoxicity is an important side-effect of this drug. Prevention and treatment of MTX-induced renal dysfunction are essential to prevent potentially life-threatening MTX-associated toxicities, Present study was undertaken to determine whether vitamin E could ameliorate methotrexate-induced oxidative renal injury in rabbit.
 Â Materials and methods: Twenty eight rabbits were randomly assigned into four groups (n=7): Group 1 (control group), Group 2 (Received 20mg/kg MTX): Group 3 (Received MTX plus vitamin E 100 mg/kg orally), group 4: (vitamin E 100 mg/kg orally). On 6th day rabbits were anesthetized and renal tissue sampled for pathologic and biochemical assessment.
 Â Results:Â Data showed that renal tissue injury index and malondialdehyde (MDA)level were lower in MTX+vitamin E group comparing to MTX group significantly (P<0.05). Renal tissue injury index and MDA were higher in MTX+vitamin E group comparing to control group significantly (P<0.05).
 Conclusion: These findings suggest that vitamin E, by improving cellular anti-oxidant defense system, reduction in lipid peroxidation production and tissue damage, appears to have a protective role in the MTX-induced oxidative injury in renal tissue, however, further studies are essential to elucidate the exact mechanisms of MTX-induced renal toxicity, and protective effect of vitamin E.
Highlights
Methotrexate (MTX) is widely used in the treatment of clinical conditions such as breast cancer, lymphoma, stomach cancer, urinary bladder cancer, psoriasis, and rheumatoid arthritis [1]
Lipid Peroxidation Assay The level of MDA, which is a major degradation product of lipid peroxidation, was significantly elevated in the renal tissue of rabbits treated with MTX (P
blood urea nitrogen (BUN) and Cr levels were higher in MTX group comparing to MTX+ Vitamin E group significantly (P
Summary
Methotrexate (MTX) is widely used in the treatment of clinical conditions such as breast cancer, lymphoma, stomach cancer, urinary bladder cancer, psoriasis, and rheumatoid arthritis [1]. Vitamin E in Methotrexate Induced Renal injury of this drug. We aimed to investigate the effect of vitamin E on renal injury after MTX administration. The present study was undertaken to determine whether vitamin E could ameliorate methotrexate-induced oxidative renal injury in rabbit. Renal tissue injury index and MDA were higher in MTX+ vitamin E group comparing to control group significantly (P
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