Protective Role of Bone Marrow Mesenchymal Stem Cells (BMSCs) Expressing miR-124 in Endometritis

Abstract

Endometritis is correlated to repeated implantation failures. MicroRNA (miRNA) participates in several inflammatory diseases and miR-124 is involved in many diseases. Bone marrow stromal cells (BMSCs) are closely connected to the regulation of inflammation. Human endometrial epithelial cells (HEECs) were cultured in vitro , assigned into control group, LPS group and BMSC group, and miR-124 overexpressing BMSCs were constructed and co-cultured with HEECs followed by analysis of HMGB1 and NF- κ B expression by Western Blot, and the proliferation and apoptosis of HEECs. In LPS group, HEECs proliferation and miR-124 decreased, apoptosis and HMGB1 increased ( P < 0.05). After co-culture with BMSCs, it can promote HEECs proliferation, inhibit apoptosis, increase miR-124, and decrease HMGB1, NF- κ b and the secretion of inflammatory factors ( P < 0.05) with more significant changes in the high miR-124 expression group. miR-124 in endometritis endometrial epithelial cells is downregulated. In conclusion, BMSCs with high expression of miR-124 can inhibit inflammation and regulate endometrial epithelial cell apoptosis by regulating HMGB1 and NF- κ B, thereby promoting endometrial epithelial cells proliferation and delaying endometritis progression.