Abstract
Objective
 Cadmium (Cd) is a very toxic and carcinogenic heavy
 metal that can cause harmful effects on human health.
 Toxicity may develop due to Cd exposure, especially in
 the kidneys. Ankaferd blood stopper (ABS) is a herbal
 mix that is used for its hemostatic properties in surgery.
 Also, ABS enhances wound and tissue healing. In this
 study, we aimed to evaluate the possible ameliorative
 effects of ABS in Cd-induced renal damage.
 Material and Method
 Thirty-two male rats were randomly divided into 4
 groups: control, Cd (cadmium chloride, 2.5 mg/kg
 single dose, ip), ABS (ABS, 1.5 ml/kg single dose ip),
 and Cd+ABS (cadmium chloride, 2.5 mg/kg single dose
 ip- ABS, 1.5 ml/kg single dose ip). At the end of the
 experiment, urea and creatinine levels were analyzed
 from the rats’ serum. In addition, total oxidant status
 (TOS), total antioxidant status (TAS) levels, superoxide
 dismutase (SOD), and glutathione peroxidase (GPx)
 activity were measured spectrophotometrically
 from renal tissues. The oxidative stress index (OSI)
 was calculated from TOS and TAS levels. Also, we
 evaluated alterations in the mRNA expression of Bcl-
 2-associated X protein (Bax), B-cell-lymphoma-2 (Bcl-
 2), silenced information regulator 1 (SIRT1), and p53
 in kidney tissue of rats by using the qRT-PCR method.
 Results
 In the Cd group, serum urea, creatinine levels, and
 tissue oxidative stress markers, TOS and OSI were
 significantly higher while Gpx activity was significantly
 lower than in the control group (p
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