Protective nature of mangiferin on oxidative stress and antioxidant status in tissues of streptozotocin-induced diabetic rats.

Periyar Selvam Sellamuthu,Sharida Fakurazi,Subban Kamalraj,Murugesan Kandasamy,Palanisamy Arulselvan

doi:10.1155/2013/750109

Abstract

Oxidative stress plays an important role in the progression of diabetes complications. The aim of the present study was to investigate the beneficial effect of oral administration of mangiferin in streptozotocin (STZ)-induced diabetic rats by measuring the oxidative indicators in liver and kidney as well as the ameliorative properties. Administration of mangiferin to diabetic rats significantly decreased blood glucose and increased plasma insulin levels. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and level of reduced glutathione (GSH) were significantly (P < 0.05) decreased while increases in the levels of lipidperoxidation (LPO) markers were observed in liver and kidney tissues of diabetic control rats as compared to normal control rats. Oral treatment with mangiferin (40 mg/kg b.wt/day) for a period of 30 days showed significant ameliorative effects on all the biochemical and oxidative parameters studied. Diabetic rats treated with mangiferin restored almost normal architecture of liver and kidney tissues, which was confirmed by histopathological examination. These results indicated that mangiferin has potential ameliorative effects in addition to its antidiabetic effect in experimentally induced diabetic rats.

Highlights

Diabetes mellitus (DM) is a series of endocrine metabolic disorders characterized by increased fasting and postprandial glucose levels as well as an insulin deficiency and/or defects of insulin action on regulation of glucose
The present study investigated the protective effect of oral administration of mangiferin isolated from S. chinensis on the vital tissues of STZ-induced diabetic rats with reference to oxidative stress indices and antioxidant defense system, with an observation to determine the possible mechanism of the antidiabetogenic action of mangiferin
Administration of mangiferin or glibenclamide to diabetic rats significantly decreased the level of blood glucose and increased the level of insulin

Summary

Introduction

Diabetes mellitus (DM) is a series of endocrine metabolic disorders characterized by increased fasting and postprandial glucose levels as well as an insulin deficiency and/or defects of insulin action on regulation of glucose. Reactive oxygen species (ROS) play a crucial role in the pathogenesis of some serious diseases/disorders, such as cancer, liver cirrhosis, cardiovascular diseases, diabetes, and inflammation associated malfunction [3]. These effects especially increased production of free radicals and its effect during diabetes are devastating and well documented [4, 5]. Streptozotocin (STZ) is frequently used to induce diabetes mellitus in experimental animal systems, and its toxic effects are produced by nitric oxide on pancreatic β-cells. The cytotoxic action of STZ is associated with the generation of ROS causing oxidative tissue damage [6]

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