Abstract
Objective To explore the protective effect and potential mechanism of rosiglitazone(RGZ)on renal ischemia reperfusion injury. Methods Sixty male SD rats were randomly divided in to sham, kidney ischemia reperfusion injury(IRI), RGZ(10 mg/kg) , RGZ(20 mg/kg), RGZ(40 mg/kg) and RGZ(80 mg/kg)groups(n=10). The model of IRI was induced by clamping the bilateral renal pedicles.ELISA was used to examine the expression of Cr, BUN, IL-8, TNF-α and IL-6 in the serum. RT-PCR was used to detect the mRNA level of IL-8, TNF- and IL-6 in the kidney. The activation of CAT, GPX and SOD were mesaured by xanthine oxidase.The expression of PPAR-γ and p-PPAR-γ were measured by Western blot. The expression of MDA was detected by TBA.The kidney pathological morphology was measured by PAS. Results Compared with the sham group, the kidney pathological injury and the expression of Cr, BUN, IL-8, TNF-α, IL-6, p-PPAR-γ and MDA in the IRI group were significantly increased with decreasing the activation of CAT, GPX and SOD. Compared with the IRI group, the kidney pathological injury and the expression of Cr, BUN, IL-8, TNF-α, IL-6, p-PPAR-γ and MDA in the RGZ group were significantly decreased with increasing the expression of p-PPAR-γ and the activation of CAT, GPX and SOD. There was no difference on the expression of PPAR-γ between three groups. Conclusions RGZ pretreatment can protect rats againest kidney ischemia-reperfusion injury. The mechanism of which is associated with suppressing inflammation and oxidative stress by activating PPAR-γ. Key words: Models, Animal; Rats; Reperfusion Injury; Kidney; Rosiglitazone
Published Version
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