Protective effects of MnTBAP and ischemia preconditioning on small-for-size graft ischemia-reper fusion injury in liver transplantation rats

Abstract

Objective To study the protective effect and the possible mechanism of MnTBAP and ischemia preconditioning on small-for-size graft ischemia-reperfusion injury in liver transplantation rats.Methods Male Sprague-Dawley rats were divided into 4 groups(n = 24 in each group)of liver transplantation models with graft volume/standard liver volume(GV/SLV)≤45%:untreated group(A),MnTBAP-treated group(B),ischemia preconditioning group(C),and MnTBAP treatment + ischemia preconditioning group(D).The expression of NF-κB and its inhibitor I-κB,TNF-α,the level of malondialdehyde(MDA),and alanine aminotransferase(ALT)level were detected at 3rd,6th,12th,and 24th h after reperfusion.The morphological changes of hepatic tissues were observed under a light microscope at different time points.Results Compared with group A,the expression of NF-κB and the levels of MDA and ALT in the grafts were significantly reduced(P＜0.01)in groups B and D,but the expression of I-κB was increased(P ＜0.05).In group C,the expression of NF-κB and the level of MDA were significantly decreased too at the 6th,12th,and 24th h after reperfusion as compared with group B(P ＜0.05),meanwhile the level of serum ALT was obviously lower in groups B,C and D than that in group A(P＜0.01).Conelusion Both MnTBAP and ischemia preconditioning could decrease the activities of NF-κB and the release of inflammatory mediators to protect the graft against ischemia/reperfusion injury via different mechanisms.But the two methods co-employed against the injury show no more effects than anyone of the two methods. Key words: Liver transplantation; Ischemia precondition; Repeffusion injury; MnTBAP