Protective effects of ischemic postconditioning on intestinal mucosa barrier in the model of hind limb crush injury in rabbits

Abstract

Objective To explore protective effects of two types of ischemic postconditioning on intestinal mucosa barrier in the model of hind limb crush injury in rabbits. Methods A rabbit hind limb crush injury model and two types of ischemic postconditioning in the models, i. e, I-post A: occluding/opening alternately (60 s/time, 3 times) common iliac artery and vein and I-post B: binding/loosing alternately (60 s/time, 3 times) the proximum of the injury hind limb, were created, and serum levels of djamine oxidase (DAO) and intestinal fatty acid-binding protein (iFABP) were determined at 2, 6, 12 and 24 h after injury. The pathological changes of ileum were examined at 24 h after injury. Results The serun levels of iFABP at 2, 6, 12 and 24 h after injury in two groups of ischemic postconditioning were as follows: for I-post A, (108.75 ±4.81), (117.04 ±3.70), (125.52 ±4.22), (109.12 ±6.95) μg/L,and I-post B, (108.62 ±3.05), (117.83 ±3.45), (127.93 ±3.86), (106.59 ±3.92) μg/L, respectively, which were significantly lower than in control group. And also there was a significant descent in serum levels of DAO at 2 and 6 h after injury in two groups [I-post A: (7.48 ±0.46), (8.69 ±0. 69) U/ml;I-post B, (7.43 ± 0. 46), ( 8.70 ± 0.70) U/ml] as compared with those in control group ( P ＜ 0. 05 ). But there was no difference between the two groups of ischemic postconditioning. There was no difference in the pathological changes of ileum among all the groups. Conclusion Ischemic postconditioning can protect intestinal mucosa barrier in the model of hind limb crush injury in rabbits. The morphological restoration of intestinal mucosa is earlier than the function recovery of intestinal mucosa barrier. Key words: Ischemic postconditioning; Crush injury; Intestinal barrier