Abstract
Objective To investigate the protective effects of cerium oxide (CeO2) nanoparticles on myocardial ischemia-reperfusion injury in rats.Methods Fifty male Sprague-Dawley rats were randomly divided into five groups:sham group,experimental group and three preconditioning groups with different particle diameter [1-10 nm group,10-25 nm group and 50 nm group (n =10 in each group)].The myocardial ischemia-reperfusion model was established successfully.The activities of superoxide dismutase (SOD) and glutathione (GSH)-Px,and the content of malondialdehyde (MDA) in myocardial tissue were determined.The morphology of myocardium was observed under a light microscope,and the apoptosis of myocardial cells was detected by using TdT-mediated dUTP nick end labeling (TUNEL) method.Results As compared with the Sham group,the activities of SOD and GSH-Px in experimental group were significantly decreased (P ＜ 0.05),while the content of MDA was increased (P ＜ 0.05).The activities of SOD and GSH-Px were increased and the content of MDA was decreased in the three different particle diameter groups as compared with the experimental group (P ＜ 0.05).The apoptosis of myocardial cells was significantly decreased in the three different particle diameter groups [(21.00 ± 3.90)％ for 1-10 nm group; (16.38 ± 3.59)％ for 10-25 nm group; and (24.20 ± 5.88)％ for 50 nm group] as compared the experimental group [(32.92 ± 7.46) ％] (P ＜ 0.05).Conclusion The CeO2 nanoparticles can protect the myocardium from ischemia-reperfusion injury by attenuating oxidative stress. Key words: CeO2 nanoparticles ; Myocardial ischemia; Reperfusion injury ; Cardioprotection
Published Version
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