Protective effects of a synthetic soybean isoflavone against oxidative damage in chick skeletal muscle cells

Abstract

The antioxidant and protective properties of a synthetic soybean isoflavone (SI) were investigated using chick skeletal (leg) muscle cells (SMC) isolated from 20-day-old Linnan yellow broiler chick embryo. Skeletal muscle cells were cultured in Dulbecco’s modified Eagle’s medium treated with 0, 12.5, 25, 50, 75 and 100 μM SI, respectively, under 80 μM H 2O 2/FeSO 4 conditions. After 24 h of incubation, SI reduced the loss of SMC under oxidative stress by H 2O 2/FeSO 4. The addition of SI significantly promoted SMC proliferation ( P < 0.01). Upon treatment with 25, 50, 75 and 100 μM SI, the activity of total superoxide dismutase in the supernatant of the media was enhanced by 17.0%, 13.0%, 13.3% and 11.9%, respectively ( P < 0.05). Compared to the control, the activity of glutathione peroxidase was significantly increased only at 25 μM concentration of SI ( P < 0.05), and the increment was 90.7%. The activity of catalase was increased by 49.2% and 49.1%, respectively, at 75 and 100 μM SI ( P < 0.01). The concentration of creatine kinase in the media was decreased by 61.6% and 60.6%, respectively, at 75 and 100 μM SI ( P < 0.01). The addition of SI did not affect the activity of lactate dehydrogenase in the media. In conclusion, the SI protected skeletal muscle cells from oxidative damage, attributed to its antioxidant activity.