Abstract
; This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40, and 80 μmol/L genistein in 50 μmol/L FeSO 4 / H 2 O 2 treated cells for 24 h. Genistein supplementation recovered the decreased activity of total superoxide dismutase induced by FeSO4/ H 2 O 2 , significantly increased glutathione peroxidase activity (p<0.05) and decreased malondialdehyde production (p<0.05). The treatment of 80 μmol/L genistein in FeSO 4 /H 2 O 2 treated cells decreased the secretion of creatine kinase (p<0.05). Fluorescence polarization values and microviscosities observed with FeSO 4 /H 2 O 2 treated cells were significantly higher than those observed with no FeSO 4 /H 2 O 2 treated cells. The addition of 80 μmol/L genistein improved the increased fluorescence polarization value (p<0.05) caused by FeSO 4 /H 2 O 2 treatment. The microviscosity value was significantly decreased by adding genistein (p<0.05). In conclusion, genistein protected skeletal muscle cells from oxidative damage by improving antioxidative status and membrane fluidity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
