Protective effect of total ginkgo flavone glycosides in lipopolysaccharide-induced acute lung injury mice

Abstract

Objective To investigate the effect of total ginkgo flavone glycosides(TFG) on acute lung injury(ALl) and its mechanism in mice. Methods Fifty male ICR mice were randomly divided into control group,ALI group and TFG 10,20,40 mg/kg groups, the ALI model was established by lipopolysaccharide (LPS) i. p. Lung dry weight and wet weight percentage were detected. Evans blue spectrophotometry were used for determination of pulmonary vascular permeability and superoxide dismutase (SOD) activity,malondialdehyde(MDA) content in lung tissue were measured. The change of pathology was observed and the expression of nuclear factor-kappaB(NF-κB) protein in lungs was detected by immunohistochemical staining. Results Compared with ALI group,TFG induced a decrease in lung weight/dry weight ratio in 40 mg/kg group,and significantly decreased the pulmonary vascular permeability. Compared with ALI group,SOD activity was increased remarkably,and MDA content was decreased in lung tissue of TFG group(all P＜0.01). HE staining showed that lung tissues in model group had obvious histopathological signs of phlegmasia,and lots of protein in alveolus were observed. In contrast, the inflammatory reactions in TFG groups were much less. Expression of GATA-3 was obviously increased in model group,while the expression decreased notably in TFG treated group. Conclusions TFG plays a protective effect in ALI mice, whose mechanism may be explained by TFG in increasing antioxidant capacity and blockade NF-κB expression. Key words: Total ginkgo flavone glycosides lAcute lung injury; Anti-oxygen; Nuclear factor-kappaB