Effect of sofren injection on acute lung injury in septic mice

Abstract

Objective To evaluate the effect of sofren injection on acute lung injury(ALI)in septic mice. Methods Ninety-six pathogen-free male Kunming mice, aged 8-10 weeks, weighing 30-35 g, were divided into 4 groups(n=24 each)using a random number table: sham operation group(group Sham), ALI group, vehicle group(group Vehicle)and sofren injection group(group Sofren). Sepsis was induced by cecal ligation and puncture.In Vehicle and Sofren groups, double distilled water and sofren injection 4 ml/kg were intraperitoneally injected, respectively, after the model was established, followed by repeated administration once every 12 h. At 12, 24 and 48 h after operation, the animals were sacrificed and lungs were removed for microscopic examination and for determination of wet to dry weight ratio(W/D ratio), malondialdehyde(MDA)content, superoxide dismutase(SOD)activity, and contents of tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6)in lung tissues(by enzyme-linked immunosorbent assay). The lung injury score(LIS)was calculated after haematoxylin and eosin staining. Results Compared with group Sham, the LIS, W/D ratio and contents of MDA, TNF-α and IL-6 were significantly increased, and the SOD activity was decreased at each time point in ALI and Vehicle groups(P 0.05). Conclusion Sofren injection can attenuate ALI in septic mice. Key words: RHODIOLA SACRA; Sepsis; Respiratory distress syndrome, adult