Protective effect of theaflavin on erythrocytes subjected to in vitro oxidative stress.

Mahejabeen Fatima,Rajesh Kumar Kesharwani,Syed Ibrahim Rizvi,Krishna Misra

doi:10.1155/2013/649759

Abstract

Antioxidant and free radical scavenging effect of black tea theaflavins has been shown in many epidemiological studies. In the present work we report the protective mechanism of tea theaflavins on biomarkers of oxidative stress, which are elevated during stress conditions. We hereby report the in vitro effect of theaflavins on erythrocyte malondialdehyde (MDA), intracellular reduced glutathione (GSH), and plasma membrane redox system (PMRS) of rats. The effect of theaflavin on PMRS has also been validated through an in silico docking simulation study using Molegro Virtual Docker (MVD). We report that theaflavins show significant protection to erythrocyte against oxidative stress induced by tert-butyl hydroperoxide (t-BHP). The findings suggest a possible protective role of theaflavins as antioxidant.

Highlights

Tea is one of the ancient and most popularly consumed beverages worldwide
The present study reports a protective effect of theaflavins on oxidation induced alteration in malondialdehyde (MDA) level, intracellular reduced glutathione (GSH) content, and plasma membrane redox system (PMRS) activity on rat erythrocytes subjected to increased oxidative stress by incubating with tert-butyl hydroperoxide (t-BHP)
The current study was designed to determine the effect of theaflavin on the biomarkers of oxidative stress: MDA, GSH, and PMRS

Summary

Introduction

Tea is one of the ancient and most popularly consumed beverages worldwide. Mostly it is consumed as green and black tea. Eukaryotic cells display an entirely distinct electron export system known as plasma membrane redox system (PMRS) which is an electron transport chain in the plasma membrane by which cells oxidize electron donors, typically NADH and/or NADPH, and transfer the resulting electrons to the extracellular acceptors. These defenses decline with age, and during the disease conditions, some reports show that the decline in antioxidant defence can be augmented by supplementation of antioxidants [16,17,18].

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Material and Methods

Results and Discussion

Conflict of Interests