Protective effect of sugar polyphenols A on radiation injury in mice

Abstract

Objective To study the radiation protection effect of sugar polyphenols A obtained by structured modification using mouse model of radiation injury induced by 137Cs γ-ray. Methods Total 42 male Kunming mice aged 4-6 weeks were randomly divided into six groups: negative control group (NCG), positive control group (PCG), irradiation control group (ICG) as well as three irradiation experimental groups. The radiation injury model was developed by once whole-body irradiation with 6.0 Gy 137Cs γ-ray. The mice in three experimental groups were fed sugar polyphenols A respectively with doses of 0.05, 0.10 and 0.20 g/kg, for 14 d before irradiation, and continuously fed with the drug for 7 d after the irradiation. After that the mice were sacrificed, and the organ index, hematological parameters, spleen colony number, bone marrow DNA content and nucleated cells in bone-marrow were tested to evaluate the effect of sugar polyphenols A. Results Compared with the ICG, the irradiation experimental groups with the sugar polyphenols A doses of 0.05, 0.10, 0.20 g/kg showed reductions in irradiation injuries of immune system and hematopoietic system, which makes the significant increases of the spleen indexes (Z=-2.364, -3.003 and -2.747, P<0.05) and the number of endogenous spleen colony (Z=-2.458, -2.141 and -2.206, P<0.05). In the groups with the doses of 0.05 and 0.2 g/kg, the number of white blood cells were significantly increased (Z=-2.652 and-2.139, P<0.05). Besides, in the group with the dose of 0.2 g/kg, the level of red blood cells (Z=-2.364, P<0.05), bone marrow DNA content (Z=-2.492, P<0.05) and nucleated cells in bone-marrow (Z=-2.492, P<0.05) were also increased compared with the ICG. Conclusions Sugar polyphenols A has the positive effects on the recovery of immune and hematopoietic function of mice through improving the organ index, endogenous spleen colony number, peripheral white blood cell number, bone marrow DNA content and nucleated cells in bone-marrow, which can reduce the injury induced by ionizing radiation. Key words: Structural modification; Sugar polyphenols A; Ionizing radiation; Hematological parameters; Endogenous spleen colony; Bone marrow DNA