Protective effect of rutin on the antioxidant genes expression in hypercholestrolemic male Westar rat

Salem S Al-Rejaie,Mohamed M Hafez,Othman A Al-Shabanah,Mohammed M Ahmed,Mohamed M Sayed-Ahmed,Abdulaziz M Aleisa,Hatem M Abuohashish,Khaled A Al-Hosaini

doi:10.1186/1472-6882-13-136

Salem S Al-Rejaie, Mohamed M Hafez + Show 6 more

Open Access

https://doi.org/10.1186/1472-6882-13-136

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Abstract

BackgroundHigh-cholesterol diet (HCD) increases the oxidative stress in different tissues leading to many diseases. Rutin (RT) is a natural flavonoid (vitamin p), which possesses an antioxidant activity with protective potential. The present study aimed to examine the potential effects of rutin on hypercholesterolemia-induced hepatotoxicity in rat.MethodsMale Wistar rats were divided into four groups: GI) control (Rat chow), GII) Rutin (0.2% in rat chow), GIII) HCD (1% cholesterol and 0.5% cholic acid in rat chow) and GIV) rutin (0.2%) + HCD.ResultsRutin in combination with HCD induced a significant protective effect against the hepatotoxicity by reducing the plasma level of alanine transaminase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL). The HCD (GII) showed a decrease in glutathione peroxidase (GPx), glutathione reductase (GR) and increase in glutathione S transferase α (GSTα), sulfiredoxin-1(Srx1), glutamate-cysteine ligase (GCL) and paraoxonase-1(PON-1) genes expression levels.ConclusionTreatment with rutin reversed all the altered genes induced by HCD nearly to the control levels. The present study concluded that the HCD feedings altered the expression levels of some genes involved in the oxidative stress pathway resulting in DNA damage and hepatotoxicity. Rutin have a hepatoprotective effect through the mechanism of enhancing the antioxidant effect via amelioration of oxidative stress genes.

Highlights

High-cholesterol diet (HCD) increases the oxidative stress in different tissues leading to many diseases
Rats fed with HCD for 6 weeks had significant increase in of AST and alanine transaminase (ALT) levels (p
The present results showed an insignificant decrease by 23% in the expression of GPX gene and significant decrease by 65% in glutathione reductase (GR) genes in rats fed with HCD compared to control group (Figure 1A & B)

Summary

Introduction

High-cholesterol diet (HCD) increases the oxidative stress in different tissues leading to many diseases. A major factor associated with these liver fatty acids depletion in obesity is the development of prolonged oxidative stress, which. Obesity is increasing worldwide especially in places with high dietary fat intake and is associated with lot of complications including NAFLD [9]. NAFLD is a common disease with an estimated prevalence in unselected population of developed nations around 20–30% [10]. Saudi Arabia has been undergoing massive developments. These developments are causing drastic changes in lifestyles and dietary habitats, like many other developed societies some of these changes tremendously increasing physical disorders such as obesity and NAFLD.

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