Protective Effect of Resveratrol on Paraoxon-Induced Damage and Apoptosis of Normal Skin Fibroblast

Abstract

Organophosphate pesticide poisoning has become a global concern in recent decades. This study aimed to explore the protective effects of resveratrol, a well-known bioactive natural product, on human normal skin fibroblasts after paraoxon treatment and its specific mechanisms. The methyl thiazolyl tetrazolium assay was used to study the effect of resveratrol on the viability of normal skin fibroblasts after paraoxon treatment. Delta alpha pi staining was performed to observe apoptosis in the normal skin fibroblasts. JC-1 staining and 2’,7’-dichlorofluorescein staining were used to investigate mitochondrial damage. The expressions of p38-mitogen-activated protein kinase and c-Jun N-terminal kinase signaling pathway-related proteins were determined using western blotting. Resveratrol enhanced the viability of normal skin fibroblasts in a dose-dependent manner after paraoxon treatment. It significantly reduced the number of cells showing nuclear concentration/fragmentation and the apoptotic level after paraoxon treatment. Moreover, resveratrol significantly lowered the 2’,7’-dichlorofluorescein fluorescence intensity in normal skin fibroblasts, decreased the JC-1 green fluorescence level, and increased the red fluorescence level in normal skin fibroblasts with increasing concentrations. Finally, resveratrol considerably reduced the expressions of phosphorylated extracellular signal-regulated protein kinases, c-Jun N-terminal kinase, and p38-mitogen-activated protein kinase. Resveratrol can alleviate paraoxon toxicity in normal skin fibroblasts by reducing apoptosis, mitigating mitochondrial damage, and inhibiting the mitogen-activated protein kinase pathway. Therefore, resveratrol may be a novel therapeutic option for organophosphate pesticide poisoning.