Protective Effect of Resveratrol Co-Administration with Cholesterol Diet on Erythrocyte Osmotic Fragility and Malondialdehyde Concentration in Rabbits

Abstract

The aim of the experiments was to investigate the protective effect of resveratrol co-administration with cholesterol diet on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in rabbits. Thirty rabbits divided into six group of five animals (n = 5) each were used for the experiment: Group 1 = normal control (C), group 2 = cholesterol diet (CD) only, group 3 = resveratrol 200 mg/kg (R200), group 4 = resveratrol 400 mg/kg (R400), group 5 = CD + R200 and group 6 = CD + R400. Eight weeks after the treatment period, blood sample of about 5 ml (3 ml in EDTA bottle for erythrocyte osmotic fragility (EOF) test and 2 ml in plane tube for extraction of serum for malondialdehyde concentration (MDA) were drawn from the heart of each sacrificed animal from all group by cardiac puncture. At 0.45% of NaCl concentration, the percentage haemolysis of 100% obtained in the CD group was considerably higher than the value of 59% recorded in the CD + R400 mg/kg and 30% haemolysis recorded for normal control group. Increases in haemolysis were indicated on the point in the graph presented. The MDA concentration obtained in the CD group rabbits (2.64±0.18 nmol/ml) was higher compared to those of CD + R200 and CD + R400 with a value of 1.70±0.14 nmol/ml and 1.64±0.12 nmol/ml respectively. It is concluded that CD increased haemolysis and MDA concentration in rabbits, ameliorated by resveratrol administration.