Protective Effect of Resveratrol and Vitamin B17 on 8-iso-Prostaglandin F2α and Raftlin-1 Levels in an Experimental Acute Urinary Retention in Rat

Abstract

Background: The incidence and complications of bladder disorders due to ischemia-reperfusion (I/R) injury constitute an important public health problem. The harmful effects of the disease could be reduced or completely eliminated by using natural antioxidant agents. In this study, the effects of preconditioning with Resveratrol and B17 vitamin, on bladder I/R damage was evaluated by the levels of 8-iso-prostaglandin F2α (8-iso-PGF2α) and raftlin-1 (RFTN-1) biomarkers. Methods: A total of 42 Wistar Albino male rats were used in the study which were divided in 6 groups (Group 1: Control, Group 2: Sham, Group 3: I/R, Group 4: Treatment 1 (RSV), Group 5: Treatment 2 (B17), Group 6: Combined treatment (RSV+B17). The effect of RSV and B17 valuated as the therapeutic group. Clamping the penile urethra of the rats, diuresis was forced and overdistension was achieved. After being kept in overdistension for 30 minutes, the rat bladder was emptied with a 3F catheter. The reperfused bladder was kept in this way for 30 minutes and the rats were sacrificed. 8-iso-PGF2α and RFTN-1 levels were measured in bladder tissue by ELISA. Result: In the I/R rat bladder tissue, we detected severe tissue injuries (p less than 0.05). As compared to the control group; It was observed that 8-iso-PGF2α and RFTN-1 values increased statistically significantly in sham, I/R and treatment 2 groups (p less than 0.05). 8-iso-PGF2α and RFTN-1 values were statistically significantly decreased in the therapeutic groups (Treatment 1, Treatment 2, Combined treatment compared to the I/R and sham group (p less than 0.05). A statistically significant decrease was observed in the treatment 1 group compared to the treatment 2 group in terms of 8-iso-PGF2α and RFTN-1 values (p less than 0.05).