Abstract
Pyrus ussuriensis Maxim (Korean pear) has been used for hundreds of years as a traditional herbal medicine for asthma, cough, and atopic dermatitis in Korea and China. Although it was originally shown to possess anti-inflammatory, antioxidant, and antiatopic properties, its gastroprotective effects have not been investigated. In the present study, we evaluated the protective effects of Pyrus ussuriensis Maxim extract (PUE) against ethanol-induced gastritis in rats. The bioactive compound profile of PUE was determined by gas chromatography mass spectroscopy (GC-MS) and high-performance liquid chromatography (HPLC). The gastroprotection of PUE at different doses (250 and 500 mg/kg body weight) prior to ethanol ingestion was evaluated using an in vivo gastritis rat model. Several endpoints were evaluated, including gastric mucosal lesions, cellular degeneration, intracellular damage, and immunohistochemical localization of leucocyte common antigen. The gastric mucosal injury and ulcer score were determined by evaluating the inflamed gastric mucosa and by histological examination. To identify the mechanisms of gastroprotection by PUE, antisecretory action and plasma prostaglandin E2 (PGE2), gastric mucosal cyclic adenosine monophosphate (cAMP), and histamine levels were measured. PUE exhibited significant antioxidant effects with IC50 values of 56.18 and 22.49 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′- azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) inhibition (%), respectively. In addition, GC/MS and HPLC analyses revealed several bioactive compounds of PUE. Pretreatment with PUE significantly (p < 0.05) decreased the ulcer index by preventing gastric mucosal lesions, erosion, and cellular degeneration. An immunohistochemical analysis revealed that PUE markedly attenuated leucocyte infiltration in a dose-dependent manner. The enhancement of PGE2 levels and attenuation of cAMP levels along with the inhibition of histamine release following PUE pretreatment was associated with the cytoprotective and healing effects of PUE. In contrast, the downregulation of the H+/K+ ATPase pathway as well as muscarinic receptor (M3R) and histamine receptor (H2R) inhibition was also involved in the gastroprotective effects of PUE; however, the expression of cholecystokinin-2 receptors (CCK2R) was unchanged. Finally, no signs of toxicity were observed following PUE treatment. Based on our results, we conclude that PUE represents an effective therapeutic option to reduce the risk of gastritis and warrants further study.
Highlights
Ethanol-induced gastritis is a major gastrointestinal (GI) disorder characterized by a condition in which the stomach mucosa becomes inflamed [1,2]
We evaluated the effects of Pyrus ussuriensis Maxim extract (PUE) in relation to the modification of biomarkers including plasma histamine and H2 -receptors, gastric mucosal cyclic adenosine monophosphate, and prostaglandin E2 (PGE2 )
We further evaluated the antioxidant activity known to be effective at preventing ethanol-induced gastritis as a biological indicator
Summary
Ethanol-induced gastritis is a major gastrointestinal (GI) disorder characterized by a condition in which the stomach mucosa becomes inflamed [1,2]. Antioxidants 2021, 10, 439 is continuously exposed to various substances that have the capacity to cause epithelial damage, it produces less mucus and other substances that normally protect the gastric mucosa from the acidic digestive fluid. Mucosal integrity is maintained by defensive factors including an epithelial barrier, blood flow, bicarbonate, mucus secretion, prostaglandins, and growth factors. Long-term alcohol intake can cause acute and chronic gastric mucosal injury [5,6]. This acute and chronic gastritis increases gastric acid secretion
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