Abstract
The goal of the present work was to investigate the protective effect of pumpkin seed oil (PSO) against lead acetate toxicity in male mice. lead acetate (20 mg/kg b.w.) was administered orally to mice once a day for 30 successive days. Whereas, PSO was administered to the mice orally at 1.5 mL/kg b.w. once a day for 30 successive days concurrently with treatment of lead acetate. The studied parameters were relative organs weights, DNA damage evaluation using comet assay in liver and kidney cells and micronucleus test in bone marrow and biochemical assessment of the liver and kidney function. Results showed that, lead acetate caused DNA damage in tested cells and significant increase in the levels of biochemical measurements. In contrast, PSO administration plus lead acetate effectively alleviated DNA damage in tested cells and improved the biochemical alterations. It can be concluded that PSO may has a protective role against lead acetate toxicity in male mice.
Highlights
In both developing and industrialized countries, environmental and occupational lead pollution is a common problem and may contribute to multi-organ toxicity in man and animals (El-Tantawy, 2016; Mohammed et al, 2017).Lead is a naturally occurring bluish-gray metal found in small amounts in the Earth’s crust and can be found in all parts of our environment (Gupta, 2007)
Oral administration of pumpkin seed oil (PSO) plus lead acetate produced a significant increase (P≤0.05) in the relative weights of liver (3.38±0.02) and kidney (1.61±0.02) compared with lead acetate treated male mice. These results are in coincidence with the findings of Abdel-Moniem et al (2011) they found that exposure to lead acetate (20 mg/kg) reduced the body and kidney weight of male rats
Hashemi (2013) reported that pretreatment with PSO increased the weight of testes in sodium valproate's treated rats
Summary
In both developing and industrialized countries, environmental and occupational lead pollution is a common problem and may contribute to multi-organ toxicity in man and animals (El-Tantawy, 2016; Mohammed et al, 2017). Lead is a naturally occurring bluish-gray metal found in small amounts in the Earth’s crust and can be found in all parts of our environment (Gupta, 2007). It is found in our food, water, air and soil. Kidney is one of targeted site of lead toxicity for being major route of excretion from body and facilitates kidney damage via oxidative stress and lipid peroxidation (Garçon et al, 2007; El-Nekeety et al, 2009)
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