Abstract
The present study was conducted to evaluate the ameliorative role of pumpkin seed oil (PSO) against potential adverse effects of bisphenol-A (BPA) in male mice. BPA was administered to the mice orally at a dose of 50 mg/kg body weight once a day for 28 successive days. While, PSO was administered to the mice orally at 1 mL/kg b.w. either before, with or after treatment of BPA, once a day for 28 successive days. The studied parameters were DNA damage evaluation using comet assay in liver and testes cells and micronucleus test in bone marrow; and histopathological examination of liver and testes tissues. Results revealed that BPA induced DNA damage in tested cells and marked histopathological alterations in liver and testes. In contrast, PSO treatments alleviated DNA damage and improved the histopathological alterations in liver and testes tissues. Furthermore, administration of mice with the PSO before BPA treatment was the best regimen in the alleviation of the adverse effects of BPA, followed by administration of PSO after then with treatment of BPA. It can be concluded that PSO may has a protective role against BPA genotoxicity and histopathological alterations in male mice.
Highlights
Among the various endocrine disrupting chemicals (EDCs), bisphenol-A (BPA) is the most important man-made compound with annual production exceeding 3.8 million tons (Michalowicz, 2014)
Growing evidence indicated that the oxidative stress caused by BPA could be one of the mechanisms leading to this toxicity (Meeker et al, 2010; Tiwari et al, 2012)
The results revealed that the main fatty acids content in pumpkin seed oil (PSO) are palmitic (17.53±2.20), stearic (7.13±0.40), oleic (14.80±0.87) and linoleic (58.23±2.31)
Summary
Among the various endocrine disrupting chemicals (EDCs), bisphenol-A (BPA) is the most important man-made compound with annual production exceeding 3.8 million tons (Michalowicz, 2014). It is a synthetic monomer used in production of polycarbonate plastics, epoxy resins, food packaging, dental sealant, water pipes, toys, fire retardant, plastic bottles, paints, pesticides and lacquers for food cans (Burridge, 2003; Fleisch et al, 2010). Growing evidence indicated that the oxidative stress caused by BPA could be one of the mechanisms leading to this toxicity (Meeker et al, 2010; Tiwari et al, 2012)
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