Protective effect of penehyclidine hydrochloride on the immune barrier of intestinal mucosa in rats with adhesive intestinal obstruction

Abstract

Objective To investigate the effect and possible mechanism of penehyclidine hydrochloride (PHC) on the immune barrier of the intestinal mucosa in rats with adhesive intestinal obstruction (AIO).Methods Sixty Sprague-Dawley (SD) rats were divided into the normal group (N group),sham-operated group (S group),normal saline group (NS group) and penehyclidine hydrochloride groups (P2,P4,and P8 groups).AIO model was established.The N group did not receive any treatment,while the Group NS and Group S received intramuscular injection of 0.4 ml normal saline,and PHC groups received intramuscular injection of 0.4 ml PHC (dosing to 0.2,0.4 and 0.8 mg/kg for Groups P2,P4 and P8 groups,respectively) for 7 days starting from day 7 after the modeling procedure.ELISA was used to measure the serum levels of IL-4 and IL-10,and the level of secretory immunoglobulin A (sIgA) in intestinal contents.Results Compared with S and NS groups,P2,P4,and P8 groups had higher serum IL-4 [(19.34±2.87),(23.85±2.72),(27.89±2.71) vs (6.18±1.42),(8.37±2.69) ng/L)],IL-10 [(118.93± 20.78),(121.61 ±24.932),(127.41 ±22.61) vs (78.19± 19.41),(42.72±21.31) ng/L] and sIgA of intestinal contents As compared to P2 groups,P4 and P8 groups had higher.IL-4 and sIgA(All P ＜ 0.05).Conclusion Moderate to high dosage of PHC can protect the immune barrier of the intestinal mucosa from injury after AIO in rats.Such protection may be related to the increased release of anti-inflammatory cytokines and suppressed production of inflammatory cytokines. Key words: Intestinal obstruction ; Intestinal mucosa; Immunity