The protective effects of penehyclidine hydrochloride on intestinal mucosa injury in rabbits with traumatic shock model

Abstract

Objective To investigate the protective effects of penehyclidine hydrochloride(PHC) on intestinal mucosa injury in rabbits with traumatic shock. Methods The animal model of traumatic shock was established according to Lamson's method.Thirty rabbits were divided into five groups randomly:control group (Con group),traumatic shock group resuscitation with normal saline (NS group),traumatic shock group resuscitation with pcnehyclidine hydrochloride in low dosages (0.05 mg/kg,PHCL group),middle dosages group (0.15 mg/kg,PHCM group) and high dosages group (0.45 mg/kg,PHCH group).At pre-shock,postshock,four time points (T3-T(o)) after resuscitation,mean arterial blood pressure(MAP) and heart rate(HR) were monitored.Plasma diamine oxidase (DAO) activity and lactic acid concentration were measured by spectrophotometry at defined time points.At the end of experiment,the animals were sacrificed and the samples were collected.The pathological changes of the samples were observed under microscope. Results There were no significant differences in MAP before shock among the five groups.MAP in NS group,PHCL group,PHCM group and PHCH group decreased post-shock,but there were no significant differences in MAP among groups.At T3-T6 time points,MAP in NS group (68.8±3.0),(67.5±5.3),(71.0±3.7),(71.0±2.4) mm Hg( 1 mm Hg=0.133 kPa),respectively was significantly lower than that before shock (94.3±1.7) mm Hg,also lower than that in the Con group (82.8±4.1),(89.8±6.6),(82A±3.3),(94.4±6.2) mm Hg,respectively and the PHCM gronp (83.2±13.6),(82.2±9.0),(83.8±8.9),(85.4±8.1) mm Hg,respectively.There were no significant differences in HR pre-shock and post-shock among the five groups.At T3-T6 time points,HR in the NS group (185±11),(181±7),(164±13),(164±13) hpm,respectively were signifieandy lower than that at T1(276±13) bpm,also significantly (P＜0.05 or P＜0.01 ) lower than that in the Con group and the PHCM group.The plasma DAO activity and D-lactate concentration in the Ns group,PHCL group,PHCM group,PHCH group were significantly increased at T2-T6 time points.Furthermore,at T3-T6 time poinls,the plasma DAO activity (0,393±0,020),(0.586±0.017),(0.844±0.036),(0.568±0.016) μ/ml,respectively and D-lactate concentration(8.292±0.364),(7.539±0.098),(5.991±0.180),(7,108±0.372) mmol/L,respectively in the NS group were significantly higher than that in the PHCL group and PHCH group; the plasma DAO activity and D-lactate concentration in the PHCL group and PHCH group were significantly higher than in the PHCM group (0.111±0.016),(0.302±0.020),(0.501±0.014),(0.183±0.018)μ/ml; (5.664±0.546),(4.609±0.292),(3.310±0,3634),(4.720±0.205)mmol/L(P＜O.05orP＜0.0l).Intestinal mucosal injury in the PHCM group was significantly reduced compared with tbat in the NS group.Conclusions The resuscitation of PHC combinition with Mnormal saline can stabilize hemodynamics in rabbits with traumatic shock.There was a significant protective effect on intestinal mucosal injury induced by traumatic shock,especially in the PHCM group.The plasma DAO activity and D-lactate concentration can be used to assess the extent of intestinal mucosa injury and barrier function. Key words: Traumatic shock; Penehyclidine hydrochloride; Plasma diamine oxidase; D-lactate; Mean arterial blood pressure