Protective effect of Moringa oleifera leaves extract against gentamicin induced hepatic and nephrotoxicity in rats

Abstract

Gentamicin-specific non-targeted induction of hepato- and nephrotoxicity is a clinical challenge in human and veterinary medicine. This study investigates the hepato- and nephroprotective effect of Moringa oleifera leaves extract on Gentamicin-induced hepatic and nephrotoxicity in rats. C- group showed negative control and had no treatment. C+ group received 80 mg/kg/day/i.p of gentamicin (GM) for 8 days and exhibited positive control . T1, T2, and T3 groups were treated with Moringa oleifera (MO) leave extract orally for 14 days at doses of 150, 300, and 600 mg/kg respectively after GM treatment. After 21 days of MO leave extract treatment, all the rats had liver and kidneys excisions. The assessment was done for the macroscopic changes in the liver and the microscopic changes and measurement of the MDA level of the liver and kidneys. The histopathological examination of the liver and kidneys shows that gentamicin increased the damage in the liver (degeneration, necrosis, and fibrosis score), kidneys (glomerular damage, degeneration, and necrosis of tubulous score), and MDA level. The application of MO extract at a dose of 600 mg/kg in gentamicin-induced rats can prevent the increase in the MDA levels as indicated by a decrease in the MDA levels in the liver and kidneys. The study results highlight the preventive role of MO leaves extract for Gentamicin induced toxicity that could be attributed to the antioxidant properties of phytochemicals.