Abstract
This research investigated the protective effect of lactobacillus plantarum against alcohol-induced liver injury and the regulatory mechanism of Keap-Nrf2-ARE signal pathway in zebrafish. Firstly, a zebrafish alcoholic liver injury model was established using1.0mM of ethanol concentration, then two forms of lactobacillus plantarum treatment were designed to perform repair, including a lactobacillus plantarum thallus suspension (LPS) and a lactobacillus plantarum thallus breaking solution (LPBS). After 24h of alcohol injury, lactobacillus plantarum concentrations of 0, 1.0×105, 1.0×106, 1.0×107 and 1.5×107 cfu/mL were added to protect zebrafish larvae. Then with the treatment of lactobacillus plantarum after 48h, activities of alanine transaminase (ALT), aspartate transaminase (AST), superoxide dismutase (SOD) and malondialdehyde (MDA) in zebrafish tissue homogenate were respectively determined. Keap-Nrf2-ARE signal pathway related gene expression conditions were also analyzed, including nuclear factor (erythroid-derived 2)-like 2(Nrf2), Kelch like ECH associated protein 1(Keap1), catalase(CAT), hemooxygenase1(HO1) and Glutathione S-Transferase Kappa 1(gstk1). Results showed that: in comparison with the control group, the LPBS with dosage of 1.0×107 cfu/mL remarkably improved the activities of SOD, CAT, HO1and gstk1 in zebrafish larvae liver (P<0.05), resulting in significant increase of the protein expression level of Nrf2 (225.78%) and suppression of Keap1 gene expression (73.67%)(P<0.01). As confirmed by the results, lactobacillus plantarum activated the Keap-Nrf2-ARE signal pathway from the level of transcription, the up-regulation of the expression quantity of Nrf2 protected the organism from oxidative stress and maximally reduced liver injury.
Highlights
Probiotic is regarded as viable bacteria or micro-ecological regulator [1] deriving from Greek languages of pro and bios, which means being conductive to life [2]
Researchers have reported that feeding lactobacillus can lead to changes in the immune state of animals, resulting in the increased host immunity, such as the activity of some immune cells, the contents of antimicrobial substances, immunoglobulin in the blood and the expression of immune factors in immune organs.[8,9], Among them lactobacillus plantarum has a certain protective effect for alcoholic liver injury [10,11], and it is already found, by mice research, that lactobacillus plantarum may be favorable for prevention and improvement of alcohol-induced fatty degeneration and injury, and can restore and improve intestinal balance, so as to lower the serum endotoxin level [12]
This research investigated the influence of the lactobacillus plantarum extract on the main antioxidant enzyme of zebrafish larvae with alcohol liver injury, and analyzed a possible regulatory mechanism based on the Keap-Nrf2-ARE signal pathway
Summary
Probiotic is regarded as viable bacteria or micro-ecological regulator [1] deriving from Greek languages of pro and bios, which means being conductive to life [2]. It is reported that lactobacillus plantarum has the efficacies of resisting against cancer, lowering cholesterol level, boosting immunity and delaying senescence, etc. Researchers have reported that feeding lactobacillus can lead to changes in the immune state of animals, resulting in the increased host immunity, such as the activity of some immune cells, the contents of antimicrobial substances, immunoglobulin in the blood and the expression of immune factors in immune organs.[8,9], Among them lactobacillus plantarum has a certain protective effect for alcoholic liver injury [10,11], and it is already found, by mice research, that lactobacillus plantarum may be favorable for prevention and improvement of alcohol-induced fatty degeneration and injury, and can restore and improve intestinal balance, so as to lower the serum endotoxin level [12]. Researches on the mechanism of lactobacillus plantarum in repairing alcoholic liver injury are limited, and related research applied to zebrafish alcoholic liver injury protection has not been reported
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