Protective Effect of Lactiplantibacillus plantarum 1201 Combined with Galactooligosaccharide on Carbon Tetrachloride-Induced Acute Liver Injury in Mice.

Zhongyue Ren,Qimeng Zhang,Huihui Lv,Hua Wei,Yalan Huo,Cuixiang Wan,Shufang Chen,Lingling Peng

doi:10.3390/nu13124441

Abstract

Acute liver injury (ALI) has a high mortality rate of approximately 20–40%, and it is imperative to find complementary and alternative drugs for treating ALI. A carbon tetrachloride (CCl4)-induced ALI mouse model was established to explore whether dietary intervention can alleviate ALI in mice. Intestinal flora, intestinal integrity, biomarkers of hepatic function, systemic inflammation, autophagy, and apoptosis signals were detected through a real-time PCR, hematoxylin-eosin staining, 16S rRNA gene sequencing, and so on. The results showed that Lactiplantibacillus plantarum 1201 had a strongly antioxidant ability, and galactooligosaccharide (GOS) could boost its growth. Based on these findings, the combination of L. plantarum 1201 and GOS, the synbiotic, was applied to prevent CCl4-induced ALI in mice. The current research proved that GOS promoted the intestinal colonization of L. plantarum 1201, and the synbiotic improved the antioxidant capacity of the host, regulated the intestinal flora, repaired the intestinal barrier, inhibited the activation of the MAPK/NF-κB pathway, and then inhibited the apoptosis and autophagy pathways, relieving inflammation and liver oxidation; thereby, the ALI of mice was alleviated. These results suggest that synbiotics may become a new research direction for liver-protecting drugs.

Highlights

The liver plays many important roles in the body, such as the detoxification of chemicals, including drugs and environmental contaminants, which, in turn, damage the liver [1]
The current research proved that GOS promoted the intestinal colonization of L. plantarum 1201, and the synbiotic improved the antioxidant capacity of the host, regulated the intestinal flora, repaired the intestinal barrier, inhibited the activation of the MAPK/NF-κB pathway, and inhibited the apoptosis and autophagy pathways, relieving inflammation and liver oxidation; thereby, the Acute liver injury (ALI) of mice was alleviated
We found that L. plantarum 1201 had an excellent antioxidant capacity, which meant that L. plantarum 1201 might reduce oxidative damage to inhibit the ALI caused by CCl4

Summary

Introduction

The liver plays many important roles in the body, such as the detoxification of chemicals, including drugs and environmental contaminants, which, in turn, damage the liver [1]. The molecular process of ALI is thought to involve complex interactions between oxidative stress, apoptosis, autophagy, and necrosis [4,5]. In the early stages of ALI, the initial purpose of the liver is to induce the generation of reactive oxygen species (ROS) in hepatocytes and endoplasmic reticulum stress, thereby triggering hepatocyte apoptosis and necrosis. If the inactivated liver cells are not cleared quickly by the phagocytic cells, such as the phagocytic Kupffer cells, the membranes of these liver cells will break down, leading to secondary necrosis [6,7,8]. A focus on reducing the oxidative damage of the liver is of great significance for the prevention of ALI

Objectives

Methods

Results

Discussion

Conclusion