Liver protective effects of Xiaoyaosan in acute liver injury in mice

Abstract

Objective To investigate the liver protective effects of Xiaoyaosan in carbon tetrachloride-induced acute liver injury in mice.Methods Thirty mice were randomly divided into a control group, a model group, and aXiaoyaosan group, with 10 mice in each group. The mice in theXiaoyaosan group were intragastrically administrated withXiaoyaosan, the mice in the remaining two groups were fed with an equal volume of distilled water. After 7 days, acute liver injury were inducedvia intraperitoneal injection of carbon tetrachloride peanut oil solution. The serum levels of alanine transaminase（ALT）, aspartate transaminase （AST）, malondialdehyde（MDA）and superoxide dismutase（SOD）were measured, and pathological changes of liver tissue was tested after 16 hours.Results The serum levels of ALT（136.46±15.75 U/Lvs. 22.96±6.23 U/L）, AST（145.37±16.39 U/Lvs. 31.89±7.26 U/L）, and MDA level in the liver tissue（17.48±3.45 nmol/mgvs. 4.22±1.08 nmol/mg）in the model group were significantly higher than those in the control group（allP〈0.01）, SOD level in the liver tissue significantly lower than that in the control group（261.60±20.29 U/mgvs. 336.73±25.34 U/mg,P〈0.01）. The serum levels of ALT（89.38±6.96 U/L,P〈0.01）, AST（119.04±20.44 U/L, P〈0.05）, MDA level（10.30±2.22 nmol/mg,P〈0.01） in the liver tissue in theXiaoyaosan group levels were significantly lower than those in the model group, and SOD level（304.77±31.71 U/mg,P〈0.01） in the liver tissue were significantly higher than that in the model group.ConclusionXiaoyaosan has liver protective effects in carbon tetrachloride-induced acute liver injury in mice. Key words: Xiaoyaosan; Drug-induced liver injury; Carbon tetrachloride