Protective effect of flaxseed oil the on diazinon-induced hepatotoxicity in male rats

Abstract

Here in, we investigated the possible hepatoprotective effects of flaxseed oil on Diazinon (DZN) induced oxidative stress in rat liver. Twenty-five adult male Wistar rats (180–200 g) were divided randomly into five groups: Control group (1 ml normal saline orally), DZN group (70 mg/kg/day, p.o.), flaxseed oil group (200 mg/kg/day, p.o.), and co- treatment groups (DZN 70 mg/kg/day plus flaxseed oil at doses of 100 and 200 mg/kg/day, respectively). Oxidative stress markers including liver and serum level of malondialdehyde (MDA), catalase (CAT), total antioxidant capacity (TAC), and total thiol group (TTG) were analyzed. Also liver function tests such as serum Alanine aminotransferases (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) were evaluated. Hematoxylin and eosin (H&E) staining was used for liver histological study. Compared to control group, DZN significantly increased liver and serum level of MDA, CAT, TAC and TTG. Also, DZN increased serum ALT, AST, and ALP levels. Flaxseed oil consumption significantly improved oxidative stress status and liver functions tests. Theses effectiveness was completely dose dependent. Also, flaxceed oil ameliorates liver histopathological alterations induced by DZN. Our data demonstrated DZN induced liver toxicity through oxidative stress mechanisms. Flaxceed supplementation had liver protective effects by decreasing oxidative stress markers and improvement of antioxidative status. Also, flaxceed reduced the development of DZN-induced alterations in liver. It seems that flaxceed supplementation may have protective role in DZN-induced oxidative damage in hepatic tissues.