Abstract
Alloxan has been widely used to produce experimental diabetes mellitus syndrome. This study was undertaken to investigate the protective effect against alloxan-induced pancreatic damage by curcumin. Rats were divided into four groups. (1) Control group. (2) Curcumin control group. (3) Diabetic group with a single intraperitoneal injection of 100mgalloxan/kgb.wt. (4) Diabetic group treated with curcumin orally at a dose of 60mg/kgb.wt dissolved in distilled water. Blood samples were collected for the detection of serum glucose levels. The pancreas was prepared for histopathological, immunohistochemical stain using anti-insulin antibody and ultrastructural studies. The electron microscopic examination of diabetic pancreas showed marked changes in pancreatic acini represented by dilated rough endoplasmic reticulum, decrease of secretory granules, cytoplasmic vacuolation and irregular contours of nuclei. β-Cells showed fusion of some granules, obvious vacuolation and pyknotic nuclei. α Cells revealed focal necrosis and vacuolation, dilated rough endoplasmic reticulum and depletion of secretory granules. Curcumin improved the histopathology and ultrastructure changes of the islets, alpha cells and exocrine acini, which nearly reverted to their normal structure. It increased insulin immunoreactivity and decreased the elevated glucose concentrations.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
