Abstract
Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. The present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. The concentration at which bergapten inhibited 50% of the cells from hemolysis (IC50) was determined on a dose-response curve, plotted as logarithmic (concentration) against percentage inhibition, keeping the hemolysis produced within the control group at 100%. Bergapten treatment produced an IC50 value of 7.71 ± 0.27 μg/ml and 4.23 ± 0.42 μg/ml for hypotonicity and heat-induced hemolysis, respectively. Diclofenac sodium at similar concentrations produced an IC50 value of 12.22 ± 0.30 μg/ml and 9.44 ± 0.23 μg/ml in the hypotonicity and heat-induced hemolysis, respectively. The ability of bergapten to inhibit protein denaturation was studied as part of an investigation on its mechanism of action. The results showed a significant concentration-dependent reduction in protein denaturation. When administered at 10, 30, and 100 μg/ml, bergapten produced a concentration-dependent reduction in albumin denaturation. Bergapten inhibited protein denaturation with IC50 values of 5.34 ± 0.30 μg/ml and 12.18 ± 0.20 μg/ml in the heat-treated egg albumin and bovine serum albumin denaturation experiments, respectively. Diclofenac sodium (10, 30, and 100 μg/ml) exhibited a similar protection against heat-treated egg albumin and bovine serum albumin denaturation experiments with IC50 values of 8.93 ± 0.17 μg/ml and 12.72 ± 0.11 μg/ml, respectively. Taken together, data from this study show that the pharmacological properties of bergapten may in part be related to its membrane-stabilizing and antidenaturation properties.
Highlights
Bergapten, a furocoumarin found in many medicinal plants, is used for the management of various conditions. e present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation
Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. e concentration at which bergapten inhibited 50% of the cells from hemolysis (IC50) was determined on a dose-response curve, plotted as logarithmic against percentage inhibition, keeping the hemolysis produced within the control group at 100%
Bergapten showed a concentration-dependent anti-inflammatory activity and protected the human erythrocyte membrane exposed to the hypotonic solution. e concentration at which 50% of the blood cells were inhibited from hemolysis (IC50) was determined on a dose-response curve, plotted as log against percentage inhibition, keeping the hemolysis produced within the control group at 100%. e IC50 value for bergapten was found at 7.71 ± 0.27 μg/ml (Figure 1)
Summary
A furocoumarin found in many medicinal plants, is used for the management of various conditions. e present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. E present in vitro study evaluated the ability of bergapten to prevent human erythrocyte hemolysis and protein denaturation. Bergapten administered at 10, 30, and 100 μg/ml exhibited a significant concentration-dependent protection on the erythrocyte membrane exposed to hypotonicity and heat-induced hemolysis. Diclofenac sodium at similar concentrations produced an IC50 value of 12.22 ± 0.30 μg/ml and 9.44 ± 0.23 μg/ml in the hypotonicity and heat-induced hemolysis, respectively. Diclofenac sodium (10, 30, and 100 μg/ml) exhibited a similar protection against heat-treated egg albumin and bovine serum albumin denaturation experiments with IC50 values of 8.93 ± 0.17 μg/ml and 12.72 ± 0.11 μg/ml, respectively. E present study, which evaluated the ability of bergapten to stabilize the human erythrocyte membrane and inhibit protein denaturation, provides further insight into the mode of anti-inflammatory action of this compound Bergapten reduced cell infiltration into lung tissue and improved histological parameters (reduced oedema, congestion, and alveolar septa thickening) in vivo. e present study, which evaluated the ability of bergapten to stabilize the human erythrocyte membrane and inhibit protein denaturation, provides further insight into the mode of anti-inflammatory action of this compound
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