Investigation of Carvedilol as an Antidenaturing and Anticataract Agent

Abstract

Cataract is an opacification of the lens of eye. Causes of cataract formation are aging, generation of free radicals, diabetes, protein denaturation by oxidative stress or any inducer etc. Antioxidants and antioxidant enzymes, antidenaturing agent protect the eyes by reducing free radical damage and eye lenses by inhibiting protein denaturation. The present study evaluated the in vitro anticataract and antidenaturing activities of carvedilol against glucose-induced cataractogenesis using goat lenses and heat induced egg albumin denaturation. Transparent isolated goat lenses are incubated in artificial aqueous humor and divided into five experimental groups. The carvedilol at a dose of 20μg/ml is incubated simultaneously with glucose (55 mM) and glucose (5.5 mM) for a period of 72 h. ascorbic acid (20 μg/ml) is used as the standard drug. At the end of the incubation lense opacity is measured by photographic evaluation. Antidenaturing activity is evaluated by taking different concentration of carvedilol (10 p.p.m – 500 p.p.m) with egg albumin and subjected for protein denaturation by using heat for 15 min. Standard drug is used as Diclofenac sodium, at the end protein denaturation is analyse by taking absorbance at 440 nm and 660 nmrsp. The carvedilol shows significant inhibition of cataractogenesis of eye lenses by carvedilol at conc. 20 p.p.m. and also thermally induced protein denaturation inhibited by carvedilol at conc. 500 p.p.m. The present supports carvedilol as an antidenaturing and anticataract agent.