Abstract

BackgroundCisplatin (CP) is an effective anticancer drug broadly used for various types of cancers, but it has shown ototoxicity that results from oxidative stress. Berberine has been reported for its anti-oxidative stress suggesting its therapeutic potential for many diseases such as colitis, diabetes, and vascular dementia.ObjectiveOrgan of Corti of postnatal day 3 mouse cochlear explants were used to compare hair cells after the treatment with cisplatin alone or with berberine chloride (BC) followed by CP.MethodsWe investigated the potential of the anti-oxidative effect of BC against the cisplatin-induced ototoxicity. We observed a reduced aberrant bundle of stereocilia in hair cells in CP with BC pre-treated group. Caspase-3 immunofluorescence and TUNEL assay supported the hypothesis that BC attenuates the apoptotic signals induced by CP. Reactive oxygen species level in the mitochondria were investigated by MitoSOX Red staining and the mitochondrial membrane potentials were compared by JC-1 assay.ResultsBC decreased ROS generation with preserved mitochondrial membrane potentials in mitochondria as well as reduced DNA fragmentation in hair cells. In summary, our data indicate that BC might act as antioxidant against CP by reducing the stress in mitochondria resulting in cell survival.ConclusionOur result suggests the therapeutic potential of BC for prevention of the detrimental effect of CP-induced ototoxicity.

Highlights

  • Hearing loss is a symptom of having an impaired perception of various tunes and sounds

  • To determine the protective effects of berberine chloride against cisplatin-induced ototoxicity in the organ of Corti, we investigated morphological defects of inner hair cells (IHCs) and outer hair cells (OHCs) by staining stereocilia with phalloidin

  • We investigated whether berberine chloride (BC) is efficient in preventing CP caused ototoxicity in postnatal day 3 mouse cochlear explants

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Summary

Introduction

Hearing loss is a symptom of having an impaired perception of various tunes and sounds. We investigated the potential of berberine chloride as a protective agent against cisplatin-induced ototoxicity in murine cochlear explants by focusing on ROS generation and apoptosis of hair cells.

Results
Conclusion
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