Abstract
Objective To investigate the protective effects of autologous adipose derived stem cells (ADSCs) on rat renal cold ischemia and reperfusion injury (IRI). Methods Adult male Wistar rats were equally randomized into group A (sham group), group B (cold IRI group), and group C (cold IRI plus immediate intra-renal administration of 2×106 autologous ADSCs, followed by intravenous autologous ADSCs at 6 hours post-reperfusion). All rats were sacrificed at 24 hours after cold IRI procedure. Results Serum creatinine levels were significantly lower in group C [(112.1±14.1) μmol/L] than in group B [(169.5±17.5) μmol/L, P=0.001]. The renal histopathologic morphology was attenuated in group C, as compared with group B. Furthermore, the mRNA expression levels of renal tissues Fas were significantly decreased in group C (0.93±0.05), and the mRNA expression levels of renal tissues heme oxgenase-1 (HO-1) were significantly increased in group C (0.35±0.03) when compared with that in group B (1.04±0.08, 0.24±0.05, respectively) (P=0.012, 0.001, respectively). Conclusion Autologous ADSCs transplantation could protect renal cold IRI by inhibiting cellular apoptosis and alleviating oxidative stress effects. Key words: Adipose derived stem cells; Renal; Cold ischemia and reperfusion injury; Autologous
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