Protective effect of autologous adipose-derived mesenchymal stem cells on renal ischemia reperfu-sion injury in rats

Abstract

Objective To observe the protective effects of autologous adipose-derived mesenchy- real stem cells （ADMSCs） on the kidney in rats during renal ischemia reperfusion （IR） injury. Methods Adult male Sprague-Dawley （SD） rats （n = 24 ） were equally randomized into group A （sham control ） , group B （IR plus culture medium only） , and group C （IR plus intravenous administration of 1.0 x 106 autologous ADMSCs 1 h after IR）. The duration of ischemia was 1 h, followed by 72 h of reperfusion be- fore the animals were sacrificed. Results After 72 h, serum creatinine levels in groups C and B were （0. 718 _＋0.：003） and （ 1. 277 _＋ 0. 011 ） mg/dL, the urine volume in group C and B was （57. 742 _＋ 0.012） and （23.665 _＋0.027） mL, the ratio of protein and creatinine in group C and B was 1.217 _＋ 0. 033 and 1. 672 _＋ 0. 013 respectively, and the comparison of all above items showed statistically significan difference （ all P 〈 0. 05 ） ; Also, apparently, the renal pathohistological lesions were aggravated in group B as compared with those in group C. Western blotting showed notably higher NAD（P） H quinone oxi- doreductase 1 （NQO1） and heine oxygenase -1 （HO-1） protein expression in group C than in group B. Conclusion ADMSCs therapy minimized kidney damage after IR injury through suppressing oxidative stress. Key words: Acute kidney disease; Adipose-derived mesenchymal stem cells; Cell transplanta- tion